Flavobacterium nakdongensis sp. nov., Isolated from Fresh Water during the Cyanobacterial Bloom Period

Abstract

A novel Gram-negative bacterial strain, 20NA77.7^T, was isolated from fresh water of the Nakdong River. Strain 20NA77.7^T shared the highest similarity with Flavobacterium indicum GPTSA100-9^T (97.91%) and Flavobacterium urocaniciphilum DSM 27078^T (96.24%) in the 16S rRNA gene sequence. The digital DNA-DNA hybridization and average nucleotide identity values for strain 20NA77.7^T with Flavobacterium species were below 20.8% and 77.33%, respectively. The major fatty acids of strain 20NA77.7^T were identified as iso-C_15:0, iso-C_16:0, iso-C_15:1 G, anteiso-C_15:0, iso-C_15:0 3OH, and iso-C_16:0 3OH. Strain 20NA77.7^T contained phosphatidylethanolamine, one unidentified aminolipid, and three unidentified lipids as polar lipids and menaquinone-6 as menaquinone. The polyphasic evidence supports the classification of strain 20NA77.7^T as a novel species belonging to the genus Flavobacterium, for which the name Flavobacterium nakdongensis is proposed. The type strain is 20NA77.7^T (= KCTC 102000^T = LMG 33137^T).

Keywords: Cyanobacterial bloom; Flavobacterium nakdongensis; novel species.