lncRNA WAC-AS1 promotes the progression of gastric cancer through miR-204-5p/HOXC8 axis

Yan Liu; Kaixuan Li; Yongjian Gao; Ye Feng; Xiaoling Zhao; Ruizhi Hou

doi:10.1016/j.tranon.2024.102139

lncRNA WAC-AS1 promotes the progression of gastric cancer through miR-204-5p/HOXC8 axis

Transl Oncol. 2024 Oct 11:50:102139. doi: 10.1016/j.tranon.2024.102139. Online ahead of print.

Authors

Yan Liu¹, Kaixuan Li², Yongjian Gao³, Ye Feng³, Xiaoling Zhao⁴, Ruizhi Hou⁵

Affiliations

¹ Department of Ultrasonography, the third Norman Bethune Hospital of Jilin university, Changchun, Jilin, China.
² Department of Gastrointestinal surgery, Tengzhou Central People's Hospital, Tengzhou, Shandong, China.
³ Department of Gastrointestinal and Colonretal Surgery, the third Norman Bethune Hospital of Jilin University, Changchun, Jilin, China.
⁴ Baoding Hospital of Beijing Children's Hospital, Capital Medical University, Hebei Key Laboratory of Infectious Disease Pathogenesis and Precise Diagnosis and Treatment, Baoding, Hebei, China. Electronic address: xiaoling.12356@163.com.
⁵ Department of Gastrointestinal and Colonretal Surgery, the third Norman Bethune Hospital of Jilin University, Changchun, Jilin, China. Electronic address: hourz@jlu.edu.cn.

PMID: 39395273
DOI: 10.1016/j.tranon.2024.102139

Abstract

LncRNAs affect tumorigenesis, and although the genesis, regulation and physiological mechanism of lncRNAs in gastric cancer (GC) have been reported, the research of lncRNAs still have a lot of value. Through comprehensive bioinformatics analysis, we screened the candidate lncRNA WAC-AS1(WAC-AS1). We analyzed WAC-AS1 expression in GC related tissues and cells using qRT-PCR. WAC-AS1's impact on GC growth and metastasis was investigated. LncRNA WC-AS-miR-204-5p-HOXC8 interaction was established through dual-luciferase reporter, FISH, RIP and RNA pull-down assay. We observed substantial upregulation in WAC-AS1 expression in cells and tissues of GC. WAC-AS1 through miR-204-5p/HOXC8 axis promoted GC proliferation, invasion, and migration. WAC-AS1 plays a cancer-promoting role for promoting the progression of GC.

Keywords: Gastric cancer; HOXC8; Mechanism; lncRNA WAC-AS1; miR-204-5p.