Truffle protein and its derived peptides exhibit sleep-promoting effects via regulation of lysosomal autophagy, neurological activity, tyrosine metabolism, and fatty acid elongation

Yujing Li; Yuxuan Liang; Chunxiu Peng; Jiashun Gong

doi:10.1016/j.ijbiomac.2024.136476

Truffle protein and its derived peptides exhibit sleep-promoting effects via regulation of lysosomal autophagy, neurological activity, tyrosine metabolism, and fatty acid elongation

Int J Biol Macromol. 2024 Oct 9:136476. doi: 10.1016/j.ijbiomac.2024.136476. Online ahead of print.

Authors

Yujing Li¹, Yuxuan Liang², Chunxiu Peng³, Jiashun Gong⁴

Affiliations

¹ College of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan 650201, China.
² Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen 518060, China; Shenzhen Key Laboratory of Food Nutrition and Health, College of Chemistry and Environmental Engineering and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China.
³ College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China. Electronic address: 1045418854@qq.com.
⁴ College of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Agro-products Processing Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650221, China. Electronic address: gong199@163.com.

PMID: 39393730
DOI: 10.1016/j.ijbiomac.2024.136476

Abstract

Black truffle (Tuber sinense) is a famous luxurious mushroom with abundant protein resources. Nevertheless, until now, no single study has explored the potential function of black truffle protein in any animal models. Thus, this study investigated the sleep-promoting effects of truffle albumin (TA) and its hydrolysate (TAH). Then, two novel sleep-enhancing peptides were explored from TAH. Our results showed that TA and TAH significantly prolonged the total sleep time and improved sleep quality of insomnia Drosophila. Additionally, two novel peptides YLDLAPL and YLRPEGDW with strong sleep-enhancing activity were explored by virtual screening and Drosophila with transgenic RNA interference (RNAi) technology. Finally, the transcriptomics analysis investigated potential mechanisms of sleep-enhancing effects in Drosophila: (1) regulation of the autophagic activity by altering the lysosomal protein; (2) up-regulation the genes in the pathway of neuroactive ligand-receptor interaction and promotion the function of neurons; (3) promotion the conversion of tyrosine into neurotransmitters; (4) regulation substrate feeding into the tricarboxylic acid (TCA) cycle and promotion free radical scavenging in neuronal cells; (5) promotion the fatty acid elongation and preservation neuronal cells avoid from oxidation.

Keywords: Drosophila melanogaster; RNAi; Sleep; Transcriptomic; Truffle protein.