PI4KB is an essential host factor for duck hepatitis a virus 1 replication and translation

Xinhong Li; Mingshu Wang; Anchun Cheng; Wei Zhang; Qiao Yang; Bin Tian; Xumin Ou; Di Sun; Yu He; Xinxin Zhao; Ying Wu; Shaqiu Zhang; Juan Huang; Zhen Wu; Yanling Yu; Ling Zhang; Dekang Zhu; Renyong Jia; Mafeng Liu; Shun Chen

doi:10.1016/j.ijbiomac.2024.136480

PI4KB is an essential host factor for duck hepatitis a virus 1 replication and translation

Int J Biol Macromol. 2024 Oct 9:136480. doi: 10.1016/j.ijbiomac.2024.136480. Online ahead of print.

Authors

Xinhong Li¹, Mingshu Wang¹, Anchun Cheng², Wei Zhang³, Qiao Yang¹, Bin Tian¹, Xumin Ou¹, Di Sun¹, Yu He¹, Xinxin Zhao¹, Ying Wu¹, Shaqiu Zhang¹, Juan Huang¹, Zhen Wu¹, Yanling Yu⁴, Ling Zhang⁴, Dekang Zhu⁵, Renyong Jia¹, Mafeng Liu¹, Shun Chen¹

Affiliations

¹ Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
² Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: chenganchun@vip.163.com.
³ Sinopharm Yangzhou VAC Biological Engineering Co., Ltd., Yangzhou 225100, China.
⁴ Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China.
⁵ Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

PMID: 39393728
DOI: 10.1016/j.ijbiomac.2024.136480

Abstract

Duck hepatitis A virus 1 (DHAV-1) is one of the most serious pathogens endangering the duck industry. Phosphatidylinositol 4-kinases (PI4Ks) are important for viral replication, and different viruses have different strategies to hijack PI4Ks. To date, few studies have investigated the DHAV-1 life cycle; thus, whether PI4Ks are required for DHAV-1 replication has not been reported. In this study, we found that the PI4KB protein, a PI4K, promoted the replication and translation of DHAV-1, and the 2A2, 2C, 2BC, 3A, 3AB, 3D, and 3CD proteins of DHAV-1 were able to interact with the PI4KB protein. Amino acids 101-120 of the 2A2 protein is the region where the 2A2 protein interacts with the PI4KB protein.

Keywords: 2A2 protein; Duck hepatitis A virus 1; Phosphatidylinositol 4-kinase beta protein.