Identification and validation of hub differential genes in pulmonary sarcoidosis

Qian Yao; Keting Min; Mengmeng Zhao; Xianqiu Chen; Dong Weng; Ying Zhou

doi:10.3389/fimmu.2024.1466029

Identification and validation of hub differential genes in pulmonary sarcoidosis

Front Immunol. 2024 Sep 19:15:1466029. doi: 10.3389/fimmu.2024.1466029. eCollection 2024.

Authors

Qian Yao^#^{1

2}, Keting Min^#², Mengmeng Zhao¹, Xianqiu Chen¹, Dong Weng¹, Ying Zhou^{1

2}

Affiliations

¹ Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
² Clinical Research Center, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

^# Contributed equally.

Abstract

A total of 138 cDEGs were screened from mediastinal lymph nodes and peripheral whole blood. Among them, 6 hub cDEGs including CTSS, CYBB, FPR2, MNDA, TLR1 and TLR8 with elevated degree and betweenness levels were illustrated in protein-protein interaction network. In comparison to healthy controls, CTSS (1.61 vs. 1.05), CYBB (1.68 vs. 1.07), FPR2 (2.77 vs. 0.96), MNDA (2.14 vs. 1.23), TLR1 (1.56 vs. 1.09), and TLR8 (2.14 vs. 0.98) displayed notably elevated expression levels within pulmonary sarcoidosis PBMC samples (P < 0.0001 for FPR2 and P < 0.05 for others), echoing with prior mRNA microarray findings. The most significant functional pathways were immune response, inflammatory response, plasma membrane and extracellular exosome, with 6 hub cDEGs distributing along these pathways. CTSS, CYBB, FPR2, MNDA, TLR1, and TLR8 could be conducive to improving the diagnostic process and understanding the underlying mechanisms of pulmonary sarcoidosis.

Keywords: hub genes; immune response; pathway enrichment analysis; protein-protein interaction network; pulmonary sarcoidosis.

MeSH terms

Gene Expression Profiling
Gene Regulatory Networks
Humans
Protein Interaction Maps*
Sarcoidosis, Pulmonary* / diagnosis
Sarcoidosis, Pulmonary* / genetics
Transcriptome

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This study has been supported by Science and Technology Innovation Research Project of Shanghai Science and Technology Commission, China (No. 20Y11902700), National Natural Science Foundation of China, China (No. 81200046, No. 82100073), and the Fundamental Research Funds for the Central Universities, China (No. 22120240364), and the Natural Science Foundation of Shanghai (22ZR1452300).