Crystallization and preliminary x-ray investigation of purine-nucleoside phosphorylase from Escherichia coli

J Biol Chem. 1985 Oct 25;260(24):12968-9.

Abstract

Crystals of purine-nucleoside phosphorylase from Escherichia coli have been grown from solutions of ammonium sulfate. The crystals are hexagonal with space group P6(1)22 or P6(5)22; the axes are alpha = 106.5 A and c = 241.3 A. The crystals are moderately stable to x-rays and diffract beyond 3.0-A resolution. It appears that the molecule, which is a hexamer, utilizes the 2-fold symmetry of the space group, resulting in three subunits/asymmetric unit.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chemical Phenomena
  • Chemistry, Physical
  • Crystallization
  • Escherichia coli / enzymology*
  • Hydrogen-Ion Concentration
  • Pentosyltransferases*
  • Purine-Nucleoside Phosphorylase*
  • X-Ray Diffraction

Substances

  • Pentosyltransferases
  • Purine-Nucleoside Phosphorylase