Comparative evaluation of gene copy number estimation techniques in genetically modified crops: insights from Southern blotting, qPCR, dPCR and NGS

Wenting Xu; Jingang Liang; Fan Wang; Litao Yang

doi:10.1111/pbi.14466

Comparative evaluation of gene copy number estimation techniques in genetically modified crops: insights from Southern blotting, qPCR, dPCR and NGS

Plant Biotechnol J. 2024 Dec;22(12):3456-3458. doi: 10.1111/pbi.14466. Epub 2024 Sep 12.

Authors

Wenting Xu¹, Jingang Liang², Fan Wang¹, Litao Yang^{1

3}

Affiliations

¹ Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
² Development Center for Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing, China.
³ Yazhou Bay Institute of Deepsea Sci-Tech, Shanghai Jiao Tong University, Sanya, China.

No abstract available

Keywords: Southern blotting; copy number estimation; digital PCR; genetically modified crops; paired‐end whole‐genome sequencing; quantitative real‐time PCR.

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