Protocol to establish an accelerated murine model for Helicobacter-induced gastric cancer

Prerna Bali; Ivonne Lozano-Pope; Jonathan Hernandez; Monica V Estrada; Christopher Benner; Marygorret Obonyo

doi:10.1016/j.xpro.2024.103302

Protocol to establish an accelerated murine model for Helicobacter-induced gastric cancer

STAR Protoc. 2024 Sep 20;5(3):103302. doi: 10.1016/j.xpro.2024.103302. Epub 2024 Sep 12.

Authors

Prerna Bali¹, Ivonne Lozano-Pope¹, Jonathan Hernandez¹, Monica V Estrada², Christopher Benner¹, Marygorret Obonyo³

Affiliations

¹ Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA, USA.
² Moores Cancer Center, University of California, San Diego, La Jolla, CA, USA.
³ Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA, USA; Moores Cancer Center, University of California, San Diego, La Jolla, CA, USA. Electronic address: mobonyo@health.ucsd.edu.

Abstract

Helicobacter-induced gastric cancer progresses very slowly, even in animal models, making it difficult to study. Here, we present a protocol to establish an accelerated murine model for Helicobacter-induced gastric cancer. We describe steps for infecting mice with Helicobacter felis, harvesting gastric tissue, assessing disease severity by histopathologic scoring, and performing gene expression studies with RT-qPCR and RNA sequencing. The accelerated model shows rapid progression of the disease, with gastric precancerous lesions developing within 6 months post-infection with Helicobacter. For complete details on the use and execution of this protocol, please refer to Bali et al.¹.

Keywords: Cancer; Microbiology; Model Organisms; Molecular Biology.

MeSH terms

Animals
Disease Models, Animal*
Female
Helicobacter Infections* / microbiology
Helicobacter Infections* / pathology
Helicobacter felis
Mice
Stomach Neoplasms* / microbiology
Stomach Neoplasms* / pathology