Encapsulation, characterization and in vitro releasing of xylanase and glucose oxidase (GOD) into cellulose nanocrystals stabilized three-layer microcapsules

Shoufeng Yang; Miaomiao Feng; Jianxiong Xu; Zilong Deng; Hongcai Zhang

doi:10.1016/j.ijbiomac.2024.135515

Encapsulation, characterization and in vitro releasing of xylanase and glucose oxidase (GOD) into cellulose nanocrystals stabilized three-layer microcapsules

Int J Biol Macromol. 2024 Sep 12;280(Pt 1):135515. doi: 10.1016/j.ijbiomac.2024.135515. Online ahead of print.

Authors

Shoufeng Yang¹, Miaomiao Feng², Jianxiong Xu¹, Zilong Deng³, Hongcai Zhang⁴

Affiliations

¹ Shanghai Veterinary Bio-tech Key Laboratory, College of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
² College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
³ State Key Laboratory for Pollution Control, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. Electronic address: zilongdeng@tongji.edu.cn.
⁴ Shanghai Veterinary Bio-tech Key Laboratory, College of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: hczhang@sjtu.edu.cn.

PMID: 39260632
DOI: 10.1016/j.ijbiomac.2024.135515

Abstract

The xylanase and glucose oxidase (GOD) are easily inactivated, restricting their applicaiton in food and agriculture fields. In this work, xylanase and glucose oxidase (GOD) were encapsulated into cellulose nanocrystals (CNC) stabilized three-layer microcapsules via ionic gelation technique to improve their bioavailability and targeted delivery. Encapsulation efficiency (EE), physicochemical properties, and in vitro releasing of xylanase and GOD encapsulated in microcapsules were investigated. EE of xylanase and GOD reached the highest values (73.34 % and 67.16 %, respectively) at an enzyme concentration of 35 mg/mL. In vitro experiments revealed that cumulative release of both enzymes encapsulated in microcapsules was greater than that of controls in simulated gastric tract (SGT) and simulated intestinal tract (SIT). The release of xylanase increased from 41.62 % (gastric tract) to 77.13 % (intestine tract), and release of GOD increased from 42.63 % to 72.11 %, respectively. Novel hydrogel carriers as enzymes encapsulation system could effectively improve the survival rate of enzymes in harsh environments and could be widely employed in food, feed and other industries.

Keywords: Cellulose nanocrystals; Encapsulation; Enzymes; In vitro releasing; Microcapsules.