An in situ ratiometric fluorescence immunosensor via Mn2+-triggered aggregation-induced emission transformation of levodopa fluorescent copolymer nanoparticles

Anal Methods. 2024 Oct 10;16(39):6676-6680. doi: 10.1039/d4ay00973h.

Abstract

Herein, a direct in situ alkaline phosphatase (ALP)-labeled luminescent nanoimmunoassay platform was constructed using Mn2+-triggered aggregation-induced emission transformation of levodopa fluorescent copolymer (LFC) nanoparticles. Using cardiac troponin I (cTn I) as the model antigen, the proposed nanoimmunosensor has been applied to detect cTn I in clinical samples with satisfactory results.

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Biosensing Techniques / methods
  • Fluorescent Dyes / chemistry
  • Humans
  • Immunoassay / methods
  • Levodopa* / chemistry
  • Manganese* / chemistry
  • Nanoparticles* / chemistry
  • Polymers* / chemistry
  • Troponin I* / analysis

Substances

  • Levodopa
  • Manganese
  • Polymers
  • Troponin I
  • Fluorescent Dyes
  • Alkaline Phosphatase