TARGET based m6A methylation-related genes predict prognosis relapsed B-cell acute lymphoblastic leukemia

BMC Pediatr. 2024 Sep 10;24(1):574. doi: 10.1186/s12887-024-05053-x.

Abstract

Purpose: The current study aims to investigate the significance of N6-methyladenosine (m6A) methylationrelated genes in the clinical prognosis of childhood relapsed B-cell acute lymphoblastic leukemia (B-ALLL) patient.

Methods: Transcriptome data and corresponding clinical data on m6A methylation-related genes (including 20 genes) were obtained from the Therapeutically Applicable Research To Generate Effective Treatments (TARGET) database.

Results: The bone marrow (BM) samples of 134 newly diagnosed (naive) and 116 relapsed B-ALL from TARGET were enrolled in the current study. Three genes (FTO, HNRNPC, RBM15B) showed significant up-regulation in relapsed B-ALL compared with that in naive B-ALL.The three genes had a significantly worse survival (P < 0.05). The LASSO Cox regression model was used to select the most predictive genes as prognostic indicators, and YTHDC1 and FTO were identified as prognostic factors for relapsed B-ALL. Finally, the results of multivariate regression analysis showed that the risk score of m6A methylation-related genes was an independent prognostic factor in relapsed B-ALL (P < 0.05).

Conclusion: We found that the expression levels of m6A methylation-related genes were different in naive and relapsed patients with B-ALL and correlated with survival and prognosis.This implies that m6A methylation-related genes may be promising prognostic indicators or therapeutic targets for relapsed B-ALL.

Keywords: Children; Prognosis; Relapsed B-ALL; m6A methylation-related genes.

MeSH terms

  • Adenosine* / analogs & derivatives
  • Adenosine* / genetics
  • Adolescent
  • Alpha-Ketoglutarate-Dependent Dioxygenase FTO* / genetics
  • Biomarkers, Tumor / genetics
  • Child
  • Child, Preschool
  • Female
  • Heterogeneous-Nuclear Ribonucleoprotein Group C / genetics
  • Humans
  • Male
  • Methylation
  • Neoplasm Recurrence, Local / genetics
  • Nerve Tissue Proteins
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma* / genetics
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma* / mortality
  • Prognosis
  • RNA Splicing Factors* / genetics
  • RNA-Binding Proteins* / genetics
  • Recurrence
  • Transcriptome
  • Up-Regulation

Substances

  • Adenosine
  • N-methyladenosine
  • RNA-Binding Proteins
  • Alpha-Ketoglutarate-Dependent Dioxygenase FTO
  • RNA Splicing Factors
  • FTO protein, human
  • YTHDC1 protein, human
  • HNRNPC protein, human
  • Heterogeneous-Nuclear Ribonucleoprotein Group C
  • RBM15 protein, human
  • Biomarkers, Tumor
  • Nerve Tissue Proteins