Chinese expert consensus on standard technical specifications for a gut microecomics laboratory (Review)

Exp Ther Med. 2024 Aug 20;28(4):403. doi: 10.3892/etm.2024.12692. eCollection 2024 Oct.

Abstract

The intestinal microbiota is a complex ecosystem that not only affects various physiological functions, such as metabolism, inflammation and the immune response, but also has an important effect on the development of tumors and response to treatment. The detection of intestinal flora enables the timely identification of disease-related flora abnormalities, which has significant implications for both disease prevention and treatment. In the field of basic and clinical research targeting gut microbiome, there is a need to recognize and understand the laboratory assays for gut microbiomics. Currently, there is no unified standard for the experimental procedure, quality management and report interpretation of intestinal microbiome assay technology. In order to clarify the process, the Tumor and Microecology Committee of China Anti-Cancer Association and the Tumor and Microecology Committee of Hubei Provincial Immunology Society organized relevant experts to discuss and put forward the standard technical specifications for gut microecomics laboratories, which provides a basis for further in-depth research in the field of intestinal microecomics.

Keywords: bioinformatics analysis; experimental procedures; gut microecology; technical specifications.

Publication types

  • Review

Grants and funding

Funding: The present study was supported by Shenzhen Nanshan District Health System Science and Technology Major Project: Induction of immune tolerance and graft-versus-host disease control program in allogeneic hematopoietic stem cell transplantation (grant no. NSZD2023018) and NHC Key Laboratory of Nuclear Technology Medical Transformation (Mianyang Central Hospital; grant no. 2023HYX034).