Preclinical pharmacology and pharmacokinetics of curcumin tagged cilostazol nanodispersion for the management of diabetic nephropathy in wister rat model

Aruna Rawat; Samrat Chauhan; Monika; Rahul Pratap Singh; Sumeet Gupta; Vikas Jhawat

doi:10.1007/s40203-024-00256-7

Preclinical pharmacology and pharmacokinetics of curcumin tagged cilostazol nanodispersion for the management of diabetic nephropathy in wister rat model

In Silico Pharmacol. 2024 Sep 2;12(2):81. doi: 10.1007/s40203-024-00256-7. eCollection 2024.

Authors

Aruna Rawat¹, Samrat Chauhan², Monika¹, Rahul Pratap Singh¹, Sumeet Gupta³, Vikas Jhawat¹

Affiliations

¹ Department of Pharmaceutical Science, School of Medical and Allied Science, GD Goenka University, Gurugram, Haryana India.
² Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab India.
³ Department of Pharmacy, Maharishi Markandeshwar (Deemed to Be) University, Mullana, Ambala, Haryana India.

PMID: 39233909
PMCID: PMC11368884 (available on 2025-09-02)
DOI: 10.1007/s40203-024-00256-7

Abstract

To evaluate the therapeutic potential of curcumin tagged cilostazol solid nano dispersion in wistar rat streptozotocin-nicotinamide-induced diabetic nephropathy. Cilostazol (CLT), a Phosphodiesterase (PDE) inhibitor has an inhibitory effect on reactive oxygen species (ROS), and Curcumin (Cur), an antioxidant, and anti-inflammatory, are water-soluble. Solid Nano dispersions were developed using the "Box-Behnken Design" and emulsion solvent evaporation procedure to improve the solubility and bioavailability. Streptozotocin (SPZ) and Nicotinamide (NA) caused diabetes in Wistar rats. DN developed 30-45 days after disease induction. All rat groups underwent histological, biochemical and pharmacokinetic evaluation. The optimized batch of Cilostazol Loaded Novel Curcumin Tagged Solid Nanodispersion (CLT-15 SND) estimated renal, lipid, and cytokine profiles better than the conventional batch. CLT-15 SND, given orally to diabetic rats for 45 days, significantly lowered fasting BGL and IL-6 levels and improved lipid and kidney-profile markers and body weight compared to plain Cilostazol Loaded Solid Nanodispersion (CLT-15 WC SND). CLT-15 SND treatment groups showed decreased blood glucose by 3.38 and 9.71 percent, increased body weight by 2.81 and 5.27 percent, improved Interleukin-6 (IL-6) by 21.36 and 18.36 percent, improved urine albumin levels by 5.67 and 14.19 percent and creatinine levels by 3.125 and 37.5 percent, improved serum urea by 30.48 percent, increased serum albumin by 2.59 and 11.18 percent, and decreased creatinine and 5.03 and 8.12 percent, respectively as compared to CLT-15 WC and MP treatment animal groups. CLT and Cur reduced IL-6, kidney, and lipid markers, demonstrating their renoprotective and pancreas-protective effects. CLT and Cur's inhibition may be the mechanism.

Keywords: Cilostazol; Curcumin; Diabetic nephropathy; Interleukin-6; Kidney profile; Lipid profile; Solid nanodispersion.

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