A versatile nanoplatform carrying cascade Pt nanozymes remodeling tumor microenvironment for amplified sonodynamic/chemo therapy of thyroid cancer

Biomaterials. 2025 Feb:313:122778. doi: 10.1016/j.biomaterials.2024.122778. Epub 2024 Aug 26.

Abstract

Thyroid cancer is increasing globally, with anaplastic thyroid carcinoma (ATC) being the most aggressive type and having a poor prognosis. Current clinical treatments for thyroid cancer present numerous challenges, including invasiveness and the necessity of lifelong medication. Furthermore, a significant portion of patients with ATC experience cancer recurrence and metastasis. To overcome this dilemma, we developed a pH-responsive biomimetic nanocarrier (CLP@HP-A) through the incorporation of Chlorin e6 (Ce6) and Lenvatinib (Len) within hollow polydopamine nanoparticles (HP) that were further modified with platinum nanoparticles (Pt), enabling synergistic chemotherapy and sonodynamic therapy. The CLP@HP-A nanocarriers exhibited specific binding with galectin-3 receptors, facilitating their internalization through receptor-mediated endocytosis for targeted drug delivery. Upon exposure to ultrasound (US) irradiation, Ce6 rapidly generated reactive oxygen species (ROS) to induce significant oxidative stress and trigger apoptosis in tumor cells. Additionally, Pt not only alleviated tumor hypoxia by catalyzing the conversion of H2O2 to oxygen (O2) but also augmented intracellular ROS levels through the production of hydroxyl radicals (•OH), thereby enhancing the efficacy of sonodynamic therapy. Moreover, Len demonstrated a potent cytotoxic effect on thyroid cancer cells through the induction of apoptosis. Transcriptomics analysis findings additionally corroborated that CLP@HP-A effectively triggered cancer cell apoptosis, thereby serving as a crucial mechanism for its cytotoxic effects. In conclusion, the integration of sonodynamic/chemo combination therapy with targeted drug delivery systems offers a novel approach to the management of malignant tumors.

Keywords: Ce6; Chemotherapy; Drug delivery carrier; Lenvatinib (Len); Sonodynamic therapy; Thyroid cancer.

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology
  • Antineoplastic Agents / therapeutic use
  • Apoptosis / drug effects
  • Cell Line, Tumor
  • Chlorophyllides*
  • Drug Carriers / chemistry
  • Humans
  • Indoles* / chemistry
  • Metal Nanoparticles / chemistry
  • Metal Nanoparticles / therapeutic use
  • Mice
  • Mice, Nude
  • Nanoparticles / chemistry
  • Phenylurea Compounds / pharmacology
  • Phenylurea Compounds / therapeutic use
  • Platinum* / chemistry
  • Platinum* / pharmacology
  • Platinum* / therapeutic use
  • Polymers* / chemistry
  • Porphyrins* / chemistry
  • Porphyrins* / pharmacology
  • Quinolines / chemistry
  • Quinolines / pharmacology
  • Reactive Oxygen Species / metabolism
  • Thyroid Neoplasms* / drug therapy
  • Thyroid Neoplasms* / metabolism
  • Thyroid Neoplasms* / pathology
  • Thyroid Neoplasms* / therapy
  • Tumor Microenvironment* / drug effects
  • Ultrasonic Therapy* / methods

Substances

  • Chlorophyllides
  • phytochlorin
  • Indoles
  • Porphyrins
  • Polymers
  • Platinum
  • Reactive Oxygen Species
  • polydopamine
  • Antineoplastic Agents
  • Phenylurea Compounds
  • Quinolines
  • Drug Carriers