Molecular features, antiviral activity, and immunological expression assessment of interferon-related developmental regulator 1 (IFRD1) in red-spotted grouper (Epinephelus akaara)

Fish Shellfish Immunol. 2024 Oct:153:109859. doi: 10.1016/j.fsi.2024.109859. Epub 2024 Aug 28.

Abstract

Interferon-related developmental regulator 1 (IFRD1) is a viral responsive gene associated with interferon-gamma. Herein, we identified the IFRD1 gene (EaIFRD1) from red-spotted grouper (Epinephelus akaara), evaluated its transcriptional responses, and investigated its functional features using various biological assays. EaIFRD1 encodes a protein comprising 428 amino acids with a molecular mass of 48.22 kDa. It features a substantial domain belonging to the interferon-related developmental regulator superfamily. Spatial mRNA expression of EaIFRD1 demonstrated the highest expression levels in the brain and the lowest in the skin. Furthermore, EaIFRD1 mRNA expression in grouper tissues exhibited significant modulation in response to immune stimulants, including poly (I:C), LPS, and nervous necrosis virus (NNV) infection. We analyzed downstream gene regulation by examining type Ⅰ interferon pathway genes following EaIFRD1 overexpression. The results demonstrated a significant upregulation in cells overexpressing EaIFRD1 compared to the control after infection with viral hemorrhagic septicemia virus (VHSV). A subcellular localization assay confirmed the nuclear location of the EaIFRD1 protein, consistent with its role as a transcriptional coactivator. Cells overexpressing EaIFRD1 exhibited increased migratory activity, enhancing wound-healing capabilities compared to the control. Additionally, under H2O2 exposure, EaIFRD1 overexpression protected cells against oxidative stress. Overexpression of EaIFRD1 also reduced poly (I:C)-mediated NO production in RAW267.4 macrophage cells. In FHM cells, EaIFRD1 overexpression significantly reduced VHSV virion replication. Collectively, these findings suggest that EaIFRD1 plays a crucial role in the antiviral immune response and immunological regulation in E. akaara.

Keywords: Antiviral activity; Epinephelus akaara; Immune response; Interferon-related developmental regulator 1; mRNA expression.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bass* / genetics
  • Bass* / immunology
  • Fish Diseases* / immunology
  • Fish Diseases* / virology
  • Fish Proteins* / genetics
  • Fish Proteins* / immunology
  • Gene Expression Profiling / veterinary
  • Gene Expression Regulation / immunology
  • Immunity, Innate* / genetics
  • Lipopolysaccharides / pharmacology
  • Nodaviridae / physiology
  • Novirhabdovirus / physiology
  • Phylogeny
  • Poly I-C / pharmacology
  • RNA Virus Infections / immunology
  • RNA Virus Infections / veterinary
  • Sequence Alignment / veterinary

Substances

  • Fish Proteins
  • Lipopolysaccharides
  • Poly I-C