In vivo induction of sugarcane (Saccharum spp.) haploids by genome editing

Plant Physiol. 2024 Oct 1;196(2):731-734. doi: 10.1093/plphys/kiae418.

Yuqiang Guo^{1

2}, Shengren Sun^{1

2

3}, Shaojiang Chen⁴, Yage Zhang⁵, Wenzhi Wang², Zuhu Deng⁶, Zhuang Liu^{1

2}, Zehuai Yu⁷, Huanyin Xu^{1

2}, Jianchun Guo⁸, Shuzhen Zhang², Yu Zhong⁴, Wei Zhang^{1

2}, Junlv Chen^{1

2}, Feng Zhou^{1

2}, Hailong Chang^{1

2}, San-Ji Gao⁶, Qinnan Wang^{1

2}

¹ Institute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, Guangdong, China.
² National Key Laboratory for Tropical Crop Breeding, Haikou 571101, Hainan, China.
³ Hainan Yazhou Bay Seed Laboratory, Sanya 572024, Hainan, China.
⁴ College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
⁵ Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya 572025, Hainan, China.
⁶ National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
⁷ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning 530004, China.
⁸ Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China.

Abstract

Mutation of the MATRILINEAL gene in sugarcane induces in vivo maternal haploids and suggests prospects for sugarcane breeding.