Surface Acoustic Wave-Enhanced Multi-View Acoustofluidic Rotation Cytometry (MARC) for Pre-Cytopathological Screening

Xiaoyan Zhang; Povilas Dumčius; Roman Mikhaylov; Jiangfa Qi; Mercedes Stringer; Chao Sun; Van Dien Nguyen; You Zhou; Xianfang Sun; Dongfang Liang; Dongge Liu; Bing Yan; Xi Feng; Changjun Mei; Cong Xu; Mingqian Feng; Yongqing Fu; Aled Clayton; Ruicong Zhi; Liangfei Tian; Zhiqiang Dong; Xin Yang

doi:10.1002/advs.202403574

Surface Acoustic Wave-Enhanced Multi-View Acoustofluidic Rotation Cytometry (MARC) for Pre-Cytopathological Screening

Adv Sci (Weinh). 2024 Aug 13:e2403574. doi: 10.1002/advs.202403574. Online ahead of print.

Authors

Xiaoyan Zhang^{1

2}, Povilas Dumčius¹, Roman Mikhaylov¹, Jiangfa Qi², Mercedes Stringer¹, Chao Sun³, Van Dien Nguyen^{4

5}, You Zhou^{4

5}, Xianfang Sun⁶, Dongfang Liang⁷, Dongge Liu⁸, Bing Yan⁹, Xi Feng¹⁰, Changjun Mei¹¹, Cong Xu¹¹, Mingqian Feng², Yongqing Fu¹², Aled Clayton¹³, Ruicong Zhi^{14

15}, Liangfei Tian¹⁶, Zhiqiang Dong², Xin Yang¹

Affiliations

¹ Department of Electrical and Electronic Engineering, School of Engineering, Cardiff University, Cardiff, CF24 3AA, UK.
² International Joint Laboratory of Biomedicine and Engineering, College of Biomedicine and Health, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.
³ School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, P. R. China.
⁴ Systems Immunity University Research Institute, Cardiff University, Cardiff, CF14 4XN, UK.
⁵ Division of Infection and Immunity, Cardiff University, Cardiff, CF14 4XN, UK.
⁶ School of Computer Science and Informatics, Cardiff University, Cardiff, CF24 4AG, UK.
⁷ Department of Engineering, University of Cambridge, Cambridge, CB2 1PZ, UK.
⁸ Department of Pathology, Beijing Hospital, Beijing, 100730, P. R. China.
⁹ Department of Information Management, Beijing Hospital, Beijing, 100730, P. R. China.
¹⁰ Department of Pathology, Hubei Cancer Hospital, Wuhan, 430079, P. R. China.
¹¹ Department of Pathology, Xiangzhou District People's Hospital of Xiangyang, Xiangyang, 441000, P. R. China.
¹² Faculty of Engineering and Environment, Northumbria University, Newcastle Upon Tyne, NE1 8ST, UK.
¹³ School of Medicine, Cardiff University, Cardiff, CF14 4XN, UK.
¹⁴ School of Computer and Communication Engineering, University of Science and Technology Beijing, Beijing, 100083, P. R. China.
¹⁵ Beijing Key Laboratory of Knowledge Engineering for Materials Science, Beijing, 100083, P.R. China.
¹⁶ Department of Biomedical Engineering, MOE Key Laboratory of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, P. R. China.

PMID: 39136049
DOI: 10.1002/advs.202403574

Abstract

Cytopathology, crucial in disease diagnosis, commonly uses microscopic slides to scrutinize cellular abnormalities. However, processing high volumes of samples often results in numerous negative diagnoses, consuming significant time and resources in healthcare. To address this challenge, a surface acoustic wave-enhanced multi-view acoustofluidic rotation cytometry (MARC) technique is developed for pre-cytopathological screening. MARC enhances cellular morphology analysis through comprehensive and multi-angle observations and amplifies subtle cell differences, particularly in the nuclear-to-cytoplasmic ratio, across various cell types and between cancerous and normal tissue cells. By prioritizing MARC-screened positive cases, this approach can potentially streamline traditional cytopathology, reducing the workload and resources spent on negative diagnoses. This significant advancement enhances overall diagnostic efficiency, offering a transformative vision for cytopathological screening.

Keywords: acoustofluidics; cell nuclear; cell rotation; cytopathology; surface acoustic wave.