The application of CRISPR-Cas system in Staphylococcus aureus infection

Heliyon. 2024 Jul 10;10(14):e34383. doi: 10.1016/j.heliyon.2024.e34383. eCollection 2024 Jul 30.

Abstract

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated nuclease (Cas) system has been proven to play an irreplaceable role in bacteria immunity activity against exogenous genetic elements. In recent years, this system has emerged as a valid gene engineering method and could be used to detect and treat various microorganisms such as bacteria and viruses, etc. Staphylococcus aureus, as a Gram-positive, opportunistic human and animal pathogen, can cause a variety of diseases greatly threatening human health. Here, we mainly reviewed the applications of the CRISPR-Cas system in Staphylococcus aureus infections in detail. Furthermore, the prospects and drawbacks of the CRISPR-Cas system were also discussed.

Keywords: CRISPR-Cas; Detection; Gene editing; Staphylococcus aureus; Treatment.