A modified auxanographic method was employed to perform carbohydrates assimilation test for 25 strains of yeasts representing the following 8 species: Candida albicans, C. glabrata, C. krusei, C. parapsilosis, C. stellatoides, C. tropicalis, Cryptococcus neoformans, and Rhodotorula glutinis. The results obtained were analyzed by comparing with the conventional auxanographic method, and it was found that the modified method was more accurate, rapid and easy in reading. The modified method was obviously easier in manipulation and more economic than that of Wickerham's broth technique and yeast assimilation agar slant technique.