Effects of pesticide exposure on the expression of selected genes in normal and cancer samples: Identification of predictive biomarkers for risk assessment

Environ Toxicol Pharmacol. 2024 Sep:110:104524. doi: 10.1016/j.etap.2024.104524. Epub 2024 Aug 5.

Abstract

Pesticides pivotal in controlling pests, can represent a threat for human health. Regulatory agencies constantly monitor their harmful effects, regulating their use. Several studies support a positive association between long-term exposure to pesticides and chronic pathologies, such as cancer. Geno-toxicological biomonitoring has proven to be valuable to assess genetic risks associated with exposure to pesticides, representing a promising tool to improve preventive measures and identify workers at higher risk. In this study, a differential gene expression analysis of 70 candidate genes deregulated upon pesticide exposure, was performed in 10 GEO human gene expression DataSets. It was found that six genes (PMAIP1, GCLM, CD36, SQSTM1, ABCC3, NR4A2) had significant AUC predictive values. Also, CD36 was upregulated in non-transformed cell samples and healthy workers, but downregulated in cancer cells. Further validation in larger groups of workers will corroborate the importance of the identified candidates as biomarkers of exposure/effect.

Keywords: Biomonitoring; Cancer; GEO DataSets; Gene expression; Genetic biomarkers; Pesticides.

MeSH terms

  • Biomarkers
  • Humans
  • Neoplasms* / chemically induced
  • Neoplasms* / genetics
  • Occupational Exposure / adverse effects
  • Occupational Exposure / analysis
  • Pesticides* / toxicity
  • Risk Assessment

Substances

  • Pesticides
  • Biomarkers