18-β-Glycyrrhetinic Acid Promotes Hair Growth by Stimulating the Proliferation of Dermal Papilla Cells and Outer Root Sheath Cells, and Extends the Anagen Phase by Inhibiting 5α-Reductase

Biol Pharm Bull. 2024;47(7):1392-1395. doi: 10.1248/bpb.b24-00302.

Abstract

18-β-Glycyrrhetinic acid, a major component of licorice, stimulated the proliferation of both dermal papilla cells and outer root sheath cells isolated from human hair follicles. Thus, suggesting that this compound promotes hair growth. Furthermore, this compound inhibited the activity of testosterone 5α-reductase, an enzyme responsible for converting androgen to dihydroandrogen, with an IC50 of 137.1 µM. 18-β-Glycyrrhetinic acid also suppressed the expression of transforming growth factor-β1 (TGF-β1), which shifts the hair cycle from the anagen phase to the telogen phase. It suggested that this compound may prolong the anagen phase. Based on these findings, this compound could be a potentially effective treatment for androgenetic alopecia.

Keywords: 18-β-glycyrrhetinic acid; 5α-reductase; dermal papilla cell; licorice; outer root sheath cell; transforming growth factor-β1 (TGF-β1).

MeSH terms

  • 3-Oxo-5-alpha-Steroid 4-Dehydrogenase / genetics
  • 3-Oxo-5-alpha-Steroid 4-Dehydrogenase / metabolism
  • 5-alpha Reductase Inhibitors* / pharmacology
  • Alopecia / drug therapy
  • Cell Proliferation* / drug effects
  • Cells, Cultured
  • Glycyrrhetinic Acid* / analogs & derivatives
  • Glycyrrhetinic Acid* / pharmacology
  • Hair / drug effects
  • Hair / growth & development
  • Hair Follicle* / cytology
  • Hair Follicle* / drug effects
  • Humans
  • Transforming Growth Factor beta1 / metabolism

Substances

  • Glycyrrhetinic Acid
  • 18alpha-glycyrrhetinic acid
  • 5-alpha Reductase Inhibitors
  • Transforming Growth Factor beta1
  • 3-Oxo-5-alpha-Steroid 4-Dehydrogenase