Yeast Expression and Activity Assay of RVFV Polymerase

Xue Wang; Lili Tang; Eiling Chen; Zhongzhou Chen

doi:10.1007/978-1-0716-3926-9_20

Yeast Expression and Activity Assay of RVFV Polymerase

Methods Mol Biol. 2024:2824:335-345. doi: 10.1007/978-1-0716-3926-9_20.

Authors

Xue Wang^#¹, Lili Tang^#¹, Eiling Chen^#², Zhongzhou Chen³

Affiliations

¹ State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing, China.
² Cherry Creek High School, Greenwood Village, CO, USA.
³ State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing, China. chenzhongzhou@cau.edu.cn.

^# Contributed equally.

PMID: 39039421
DOI: 10.1007/978-1-0716-3926-9_20

Abstract

Rift Valley fever (RVF) virus is widespread worldwide and poses a severe threat to human life and property. RVF viral polymerase plays a vital role in the replication and transcription of the virus. Here, we describe how to express and purify this polymerase and perform tests for its in vitro activity assays.

Keywords: Endonuclease; Expression; In vitro; Polymerase; Purification; RVF; Replication.

MeSH terms

Enzyme Assays / methods
Gene Expression
Humans
Rift Valley fever virus* / genetics
Saccharomyces cerevisiae / genetics
Viral Proteins / genetics
Viral Proteins / metabolism
Virus Replication / genetics

Substances

Viral Proteins