Fluorescent probes with specific and rapid response to fluoride ions are important mediators for detecting fluoride ions in biological systems. In this study, a phenothiazine-based fluorescent probe, PTC, was designed and synthesized, which undergoes cleavage activation and cyclization induced by fluoride ions targeting Si-O bonds. The probe exhibits strong anti-interference properties and reaches peak fluorescence within 5 min, allowing for quantitative detection of fluoride ions content in the concentration range of 0 to 12.5μM, suitable for live cell fluorescence imaging. The research findings suggest its potential application value in biological systems.
Keywords: Cell imaging; Fluorescence; Fluoride; Phenothiazine; Probe.
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