Digested galactoglucomannan mitigates oxidative stress in human cells, restores gut bacterial diversity, and provides chemopreventive protection against colon cancer in rats

Int J Biol Macromol. 2024 Oct;277(Pt 1):133986. doi: 10.1016/j.ijbiomac.2024.133986. Epub 2024 Jul 20.

Abstract

Galactoglucomannan (GGM) is the predominant hemicellulose in coniferous trees, such as Norway spruce, and has been used as a multipurpose emulsifier in the food industry. In vitro digestion with a cellular antioxidant activity assay was performed to determine the bioaccessibility and antioxidant activity of phenolic compounds, and the behaviour of GGM on in vivo experimental assay against induced colon cancer. The results showed that digestion decreased the bioaccessibility and antioxidant capacity of phenolic compounds. Cellular analysis did not support these findings once an antioxidant effect was observed in human cell lines. GGM attenuated the initiation and progression of colon cancer, by reducing the foci of aberrant crypts in rats, and modified the intestinal bacterial microbiota (disrupting the balance between Firmicutes and Bacteroidetes phyla). Thus, GGM provided chemopreventive protection against the development of colon cancer and acted as an intracellular antioxidant agent.

Keywords: Bioaccessibility; Erythrocytes; Hemicellulose.

MeSH terms

  • Animals
  • Antioxidants* / pharmacology
  • Cell Line, Tumor
  • Colonic Neoplasms* / metabolism
  • Colonic Neoplasms* / pathology
  • Colonic Neoplasms* / prevention & control
  • Gastrointestinal Microbiome* / drug effects
  • Humans
  • Male
  • Mannans* / chemistry
  • Mannans* / pharmacology
  • Oxidative Stress* / drug effects
  • Rats

Substances

  • Antioxidants
  • galactoglucomannan
  • Mannans