Escherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector

Mayra Marquez; Qian Chen; Silvia Cachaco; Jun Yang; Hongyan Sui; Tomozumi Imamichi

doi:10.1016/j.mimet.2024.106990

Escherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector

J Microbiol Methods. 2024 Sep:224:106990. doi: 10.1016/j.mimet.2024.106990. Epub 2024 Jul 14.

Authors

Mayra Marquez¹, Qian Chen¹, Silvia Cachaco¹, Jun Yang¹, Hongyan Sui¹, Tomozumi Imamichi²

Affiliations

¹ Laboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA.
² Laboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA. Electronic address: timamichi@mail.nih.gov.

PMID: 39004285
PMCID: PMC11330712 (available on 2025-09-01)
DOI: 10.1016/j.mimet.2024.106990

Abstract

The pUC-derived plasmid yield from E. coli using polypropylene tubes (PP) was compared among round and conical tubes. The yield from cells grown in a cheaper conical-PP with flat-bottom was 1.5-fold higher (p < 0.001) than other PP. The use of the conical-PP can save research budgets in the current inflationary environment.

Keywords: Conical tubes; Isolation; Laboratory budget; Plasmid DNA; Polypropylene; Polystyrene.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Escherichia coli* / genetics
Escherichia coli* / growth & development
Genetic Vectors
Plasmids* / genetics
Polypropylenes* / chemistry

Substances

Polypropylenes

Abstract

Publication types

MeSH terms

Substances

Grants and funding