Multienzyme Active Nanozyme for Efficient Sepsis Therapy through Modulating Immune and Inflammation Inhibition

ACS Appl Mater Interfaces. 2024 Jul 17;16(28):36047-36062. doi: 10.1021/acsami.4c04994. Epub 2024 Jul 9.

Abstract

Sepsis, a life-threatening condition caused by a dysregulated immune response to infection, leads to systemic inflammation, immune dysfunction, and multiorgan damage. Various oxidoreductases play a very important role in balancing oxidative stress and modulating the immune response, but they are stored inconveniently, environmentally unstable, and expensive. Herein, we develop multifunctional artificial enzymes, CeO2 and Au/CeO2 nanozymes, exhibiting five distinct enzyme-like activities, namely, superoxide dismutase, catalase, glutathione peroxidase, peroxidase, and oxidase. These artificial enzymes have been used for the biocatalytic treatment of sepsis via inhibiting inflammation and modulating immune responses. These nanozymes significantly reduce reactive oxygen species and proinflammatory cytokines, achieving multiorgan protection. Notably, CeO2 and Au/CeO2 nanozymes with enzyme-mimicking activities can be particularly effective in restoring immunosuppression and maintaining homeostasis. The redox nanozyme offers a promising dual-protective strategy against sepsis-induced inflammation and organ dysfunction, paving the way for biocatalytic-based immunotherapies for sepsis and related inflammatory diseases.

Keywords: cerium oxide; gold-doped cerium oxide; immune response; inflammation; nanozyme; sepsis.

MeSH terms

  • Animals
  • Catalase / chemistry
  • Catalase / metabolism
  • Cerium* / chemistry
  • Cerium* / therapeutic use
  • Cytokines / metabolism
  • Gold* / chemistry
  • Humans
  • Inflammation* / drug therapy
  • Inflammation* / immunology
  • Mice
  • Reactive Oxygen Species / metabolism
  • Sepsis* / drug therapy
  • Sepsis* / immunology

Substances

  • Gold
  • ceric oxide
  • Cerium
  • Reactive Oxygen Species
  • Catalase
  • Cytokines