Trypanosoma cruzi: metabolic labeling of trypomastigote sialoglycolipids

Experientia. 1985 Jun 15;41(6):736-8. doi: 10.1007/BF02012572.

Abstract

Trypomastigote forms (infective) of Trypanosoma cruzi incorporate (3H)-palmitic acid and D-(3H)-galactose into glycolipids. Palmitic acid-labeled acidic glycolipids were partially hydrolyzed with neuraminidase. The labeling of these compounds when the intact cell surface was labeled with galactose oxidase plus NaB3H4 indicates the membrane location of the sialoglycolipids.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Borohydrides
  • Cell Membrane / metabolism
  • Galactose / metabolism
  • Galactose Oxidase / metabolism
  • Glycolipids / metabolism*
  • Kinetics
  • Palmitic Acid
  • Palmitic Acids / metabolism
  • Tritium
  • Trypanosoma cruzi / growth & development
  • Trypanosoma cruzi / metabolism*

Substances

  • Borohydrides
  • Glycolipids
  • Palmitic Acids
  • sialoglycolipids
  • Tritium
  • Palmitic Acid
  • sodium borohydride
  • Galactose Oxidase
  • Galactose