Rapid and accurate identification of yeast subspecies by MALDI-MS combined with a cell membrane disruption reagent

Nan Zhao; Wei Guo; Jiarui Li; Hao Wang; Xinhua Guo

doi:10.1016/j.foodchem.2024.140102

Rapid and accurate identification of yeast subspecies by MALDI-MS combined with a cell membrane disruption reagent

Food Chem. 2024 Nov 1:457:140102. doi: 10.1016/j.foodchem.2024.140102. Epub 2024 Jun 15.

Authors

Nan Zhao¹, Wei Guo², Jiarui Li¹, Hao Wang³, Xinhua Guo⁴

Affiliations

¹ State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.
² Department of Nuclear Medicine, The Second Hospital of Jilin University, Changchun 130041, China.
³ Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: wangh@ciac.ac.cn.
⁴ State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China; Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China. Electronic address: guoxh@jlu.edu.cn.

PMID: 38905823
DOI: 10.1016/j.foodchem.2024.140102

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been widely used for microbial analysis. However, due to the impenetrable shell of fungi the direct identification of fungi remains challenges. Targeting on this problem, the yeast Saccharomyces cerevisiae (S. cerevisiae) was selected as a model fungus, and a new fungal cell membrane disruption reagent C₁₈-G₁ was used before MALDI-MS detection. As a result, much more intensive peaks which distributed in wider m/z range of S. cerevisiae have been identified in comparison with the use of traditional fungal pretreatment methods. Furthermore, a differential peak at m/z 4993 between two subspecies of S. cerevisiae has been identified. The corresponding protein with exclusive sequence of the specific peak was obtained based on MS/MS fragments and database searching. In addition, the method was successfully applied for the discrimination of four commercial yeasts.

Keywords: MALDI-MS; S. cerevisiae; Yeast disruption reagent; Yeast identification.

Publication types

Evaluation Study

MeSH terms

Cell Membrane* / chemistry
Saccharomyces cerevisiae* / chemistry
Saccharomyces cerevisiae* / classification
Saccharomyces cerevisiae* / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / methods