Arabidopsis cryptochromes interact with SOG1 to promote the repair of DNA double-strand breaks

Li Chen; Minqing Liu; Yupeng Li; Yan Guan; Jiaqi Ruan; Zhilei Mao; Wenxiu Wang; Hong-Quan Yang; Tongtong Guo

doi:10.1016/j.bbrc.2024.150233

Arabidopsis cryptochromes interact with SOG1 to promote the repair of DNA double-strand breaks

Biochem Biophys Res Commun. 2024 Sep 10:724:150233. doi: 10.1016/j.bbrc.2024.150233. Epub 2024 Jun 7.

Authors

Li Chen¹, Minqing Liu², Yupeng Li¹, Yan Guan¹, Jiaqi Ruan², Zhilei Mao¹, Wenxiu Wang¹, Hong-Quan Yang¹, Tongtong Guo³

Affiliations

¹ Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China; Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.
² Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.
³ Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China; Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China. Electronic address: ttguo@shnu.edu.cn.

PMID: 38865814
DOI: 10.1016/j.bbrc.2024.150233

Abstract

Cryptochromes (CRYs) are blue light (BL) photoreceptors to regulate a variety of physiological processes including DNA double-strand break (DSB) repair. SUPPRESSOR OF GAMMA RADIATION 1 (SOG1) acts as the central transcription factor of DNA damage response (DDR) to induce the transcription of downstream genes, including DSB repair-related genes BRCA1 and RAD51. Whether CRYs regulate DSB repair by directly modulating SOG1 is unknown. Here, we demonstrate that CRYs physically interact with SOG1. Disruption of CRYs and SOG1 leads to increased sensitivity to DSBs and reduced DSB repair-related genes' expression under BL. Moreover, we found that CRY1 enhances SOG1's transcription activation of DSB repair-related gene BRCA1. These results suggest that the mechanism by which CRYs promote DSB repair involves positive regulation of SOG1's transcription of its target genes, which is likely mediated by CRYs-SOG1 interaction.

Keywords: Blue light; Cryptochromes; DNA double-strand break; SOG1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis Proteins* / genetics
Arabidopsis Proteins* / metabolism
Arabidopsis* / genetics
Arabidopsis* / metabolism
Cryptochromes* / genetics
Cryptochromes* / metabolism
DNA Breaks, Double-Stranded*
DNA Repair*
Gene Expression Regulation, Plant
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

Arabidopsis Proteins
Cryptochromes
SOG1 protein, Arabidopsis
CRY1 protein, Arabidopsis
Transcription Factors