Liposome-encapsulated cytochrome P450 and gibberellic acid biosynthesis in Priestia megaterium RP1

Abstract

This study explores the potential of liposome encapsulated silica immobilized cytochrome P450 monooxygenase (LSICY) for bioremediation of mercury (Hg²⁺). Current limitations in Hg²⁺ reduction, including sensitivity to factors like pH and cost, necessitate alternative methods. We propose LSICY as a solution, leveraging the enzymatic activities of cytochrome P450 monooxygenase (CYPM) for Hg²⁺ reduction through hydroxylation and oxygenation. Our investigation employs LSICY to assess its efficacy in mitigating Hg²⁺ toxicity in Oryza sativa (rice) plants. Gas chromatography confirmed gibberellic acid (GA) presence in the Hg²⁺ reducing bacteria Priestia megaterium RP1 (PMRP1), highlighting a potential link between CYP450 activity and plant health. This study demonstrates the promise of LSICY as a sustainable and effective approach for Hg²⁺ bioremediation, promoting a safer soil environment.

Keywords: Cytochrome P450 monooxygenase; Gibberellic acid; Liposome encapsulation; Mercury bioremediation; Oryza sativa.