Highly siderophile element abundances and Os isotopes of nakhlite and chassignite meteorites demonstrate that they represent a comagmatic suite from Mars. Nakhlites experienced variable assimilation of >2-billion-year-old altered high Re/Os basaltic crust. This basaltic crust is distinct from the ancient crust represented by meteorites Allan Hills 84001 or impact-contaminated Northwest Africa 7034/7533. Nakhlites and chassignites that did not experience crustal assimilation reveal that they were extracted from a depleted lithospheric mantle distinct from the deep plume source of depleted shergottites. The comagmatic origin for nakhlites and chassignites demonstrates a layered martian interior comprising ancient enriched basaltic crust derived from trace element-rich shallow magma ocean cumulates, a variably metasomatized mantle lithosphere, and a trace element-depleted deep mantle sampled by plume magmatism.