Deinococcus arenicola sp. nov., a novel radiation-resistant bacterium isolated from sandy soil in Antarctica

Int J Syst Evol Microbiol. 2024 May;74(5). doi: 10.1099/ijsem.0.006397.

Abstract

A Gram-stain-positive, aerobic, non-mobile and spherical strain, designated ZS9-10T, belonging to the genus Deinococcus was isolated from soil sampled at the Chinese Zhong Shan Station, Antarctica. Growth was observed in the presence of 0-4 % (w/v) NaCl, at pH 7.0-8.0 and at 4-25 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZS9-10T formed a lineage in the genus Deinococcus. It exhibited highest sequence similarity (97.4 %) to Deinococcus marmoris DSM 12784T. The major phospholipids of ZS9-10T were unidentified phosphoglycolipid, unidentified glycolipids and unidentified lipids. The major fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C16 : 0 and C16 : 1 ω7c. MK-8 was the predominant respiratory quinone. The digital DNA-DNA hybridization and average nucleotide identity values between strain ZS9-10T and its close relative D. marmoris DSM 12784T were 27.4 and 83.9 %, respectively. Based on phenotypic, phylogenetic and genotypic data, a novel species, named Deinococcus arenicola sp. nov., is proposed. The type strain iis ZS9-10T (=CCTCC AB 2019392T=KCTC43192T).

Keywords: Antarctic soil; Deinococcus; new taxa; taxonomy.

MeSH terms

  • Antarctic Regions
  • Bacterial Typing Techniques*
  • Base Composition*
  • DNA, Bacterial* / genetics
  • Deinococcus* / classification
  • Deinococcus* / genetics
  • Deinococcus* / isolation & purification
  • Fatty Acids* / analysis
  • Fatty Acids* / chemistry
  • Nucleic Acid Hybridization*
  • Phospholipids* / analysis
  • Phospholipids* / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S* / genetics
  • Sand / microbiology
  • Sequence Analysis, DNA*
  • Soil Microbiology*
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / analysis
  • Vitamin K 2 / chemistry

Substances

  • RNA, Ribosomal, 16S
  • Fatty Acids
  • DNA, Bacterial
  • Phospholipids
  • Vitamin K 2
  • vitamin MK 8
  • Sand