Visualizing the spatial distribution of ustalic acid in the fruiting body of Tricholoma kakishimeji

Tetsuro Ito; Syu Taira; Wataru Aoki; Hiroyuki Nagai; Masashi Fukaya; Kaori Ryu; Akiyoshi Yamada

doi:10.1007/s11418-024-01823-0

Visualizing the spatial distribution of ustalic acid in the fruiting body of Tricholoma kakishimeji

J Nat Med. 2024 Sep;78(4):838-843. doi: 10.1007/s11418-024-01823-0. Epub 2024 May 18.

Authors

Tetsuro Ito¹, Syu Taira², Wataru Aoki³, Hiroyuki Nagai⁴, Masashi Fukaya⁵, Kaori Ryu⁵, Akiyoshi Yamada^{6

7}

Affiliations

¹ Faculty of Pharmacy, Gifu University of Medical Science, 4-3-3 Nijigaoka, Kani, Gifu, 509-0293, Japan. tito@u-gifu-ms.ac.jp.
² Faculty of Food and Agricultural Sciences, Fukushima University, 1 Kanayagawa, Fukushima, Japan.
³ Department of Agriculture Graduate School of Science and Technology, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano, 399-4598, Japan.
⁴ Gifu Prefectural Research Institute for Health and Environmental Sciences, Naka-Fudogaoka, Kakamigahara, Gifu, 504-0838, Japan.
⁵ Faculty of Pharmacy, Gifu University of Medical Science, 4-3-3 Nijigaoka, Kani, Gifu, 509-0293, Japan.
⁶ Department of Agriculture Graduate School of Science and Technology, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano, 399-4598, Japan. akiyosh@shinshu-u.ac.jp.
⁷ Department of Mountain Ecosystem, Institute for Mountain Science, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano, 399-4598, Japan. akiyosh@shinshu-u.ac.jp.

PMID: 38762660
DOI: 10.1007/s11418-024-01823-0

Abstract

Imaging mass spectrometry (IMS) was conducted for the first time using ustalic acid (UA) and the fruiting body of Tricholoma kakishimeji to localize mushroom toxins. The mushroom materials were systematically collected in Japan, and analysis of the cross sections of the materials at a resolution of 120 μm using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-IMS) revealed the localization of UA and its biogenically related metabolites. MALDI-IMS confirmed that UA was predominantly located on the entire surface of the fruiting body and accumulated in higher amounts in younger fruiting bodies than in mature ones. UA is the first toxic secondary metabolite in the genus Tricholoma locally identified using IMS in mushrooms.

Keywords: Tricholoma kakishimeji; Fruiting body; Imaging mass spectrometry; Mushroom toxin; Ustalic acid.

MeSH terms

Fruiting Bodies, Fungal* / chemistry
Japan
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
Tricholoma* / chemistry

Grants and funding

23K06201/Japan Society for the Promotion of Science