Antinuclear antibodies and nuclear antigens in NZB myeloma ascitic fluids

Clin Immunol Immunopathol. 1985 Jun;35(3):337-45. doi: 10.1016/0090-1229(85)90094-7.

Abstract

Approximately 5-10% of ascitic fluids from 411 NZB myeloma tumors were found to possess either antinuclear (ANA) or Coombs antibodies. Some fluids showed anti-SM specificity, which is thought to be unique to systemic lupus erythematosus (SLE). In addition, the ascitic fluids were found to contain large amounts of DNA and Sm autoantigens. Further passage of ANA-positive myelomas indicated that the autoantibodies were not products of the myeloma cells themselves. The sporadic appearance of unsuspected autoantibodies in ascitic fluids may cause confusion when working with myeloma or with hybridoma reagents. Furthermore, the development of SLE-specific anti-Sm antibodies in this context suggests parallels between myeloma development and autoimmunity.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Antinuclear / analysis*
  • Antigens / analysis
  • Antigens, Nuclear
  • Ascitic Fluid / immunology
  • Autoantigens
  • Hybridomas / immunology
  • Mice
  • Mice, Inbred NZB
  • Mice, Inbred Strains
  • Multiple Myeloma / immunology*
  • Nucleoproteins / analysis*
  • Ribonucleoproteins, Small Nuclear*
  • snRNP Core Proteins

Substances

  • Antibodies, Antinuclear
  • Antigens
  • Antigens, Nuclear
  • Autoantigens
  • Nucleoproteins
  • Ribonucleoproteins, Small Nuclear
  • snRNP Core Proteins