The gut commensal Blautia maintains colonic mucus function under low-fiber consumption through secretion of short-chain fatty acids

Nat Commun. 2024 Apr 25;15(1):3502. doi: 10.1038/s41467-024-47594-w.

Abstract

Beneficial gut bacteria are indispensable for developing colonic mucus and fully establishing its protective function against intestinal microorganisms. Low-fiber diet consumption alters the gut bacterial configuration and disturbs this microbe-mucus interaction, but the specific bacteria and microbial metabolites responsible for maintaining mucus function remain poorly understood. By using human-to-mouse microbiota transplantation and ex vivo analysis of colonic mucus function, we here show as a proof-of-concept that individuals who increase their daily dietary fiber intake can improve the capacity of their gut microbiota to prevent diet-mediated mucus defects. Mucus growth, a critical feature of intact colonic mucus, correlated with the abundance of the gut commensal Blautia, and supplementation of Blautia coccoides to mice confirmed its mucus-stimulating capacity. Mechanistically, B. coccoides stimulated mucus growth through the production of the short-chain fatty acids propionate and acetate via activation of the short-chain fatty acid receptor Ffar2, which could serve as a new target to restore mucus growth during mucus-associated lifestyle diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / metabolism
  • Adult
  • Animals
  • Clostridiales / metabolism
  • Colon* / metabolism
  • Colon* / microbiology
  • Dietary Fiber* / metabolism
  • Fatty Acids, Volatile* / metabolism
  • Fecal Microbiota Transplantation
  • Female
  • Gastrointestinal Microbiome*
  • Humans
  • Intestinal Mucosa* / metabolism
  • Intestinal Mucosa* / microbiology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mucus / metabolism
  • Propionates / metabolism
  • Receptors, Cell Surface*
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism
  • Symbiosis

Substances

  • Dietary Fiber
  • Fatty Acids, Volatile
  • Ffar2 protein, mouse
  • Receptors, G-Protein-Coupled
  • FFA2R protein, human
  • Propionates
  • Acetates
  • Receptors, Cell Surface