Role of Rmd9p in 3'-end processing of mitochondrial 15S rRNA in Saccharomyces cerevisiae

Mitochondrion. 2024 May:76:101876. doi: 10.1016/j.mito.2024.101876. Epub 2024 Apr 8.

Abstract

Ribosome biogenesis, involving processing/assembly of rRNAs and r-proteins is a vital process. In Saccharomyces cerevisiae mitochondria, ribosomal small subunit comprises 15S rRNA (15S). While the 15S 5'-end processing uses Ccm1p and Pet127p, the mechanisms of the 3'-end processing remain unclear. We reveal involvement of Rmd9p in safeguarding/processing 15S 3'-end. Rmd9p deficiency results in a cleavage at a position 183 nucleotides upstream of 15S 3'-end, and in the loss of the 3'-minor domain. Rmd9p binds to the sequences in the 3'-end region of 15S, and a genetic interaction between rmd9 and dss1 indicates that Rmd9p regulates/limits mtEXO activity during the 3'-end spacer processing.

Keywords: Dss1p; Mitochondrial translation; RNA processing; Ribosome; Rmd9p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Mitochondria / genetics
  • Mitochondria / metabolism
  • RNA 3' End Processing
  • RNA Processing, Post-Transcriptional
  • RNA, Fungal / genetics
  • RNA, Fungal / metabolism
  • RNA, Ribosomal* / genetics
  • RNA, Ribosomal* / metabolism
  • Saccharomyces cerevisiae Proteins* / genetics
  • Saccharomyces cerevisiae Proteins* / metabolism
  • Saccharomyces cerevisiae* / genetics
  • Saccharomyces cerevisiae* / metabolism

Substances

  • RNA, Fungal
  • RNA, Ribosomal
  • Saccharomyces cerevisiae Proteins
  • RMD9 protein, S cerevisiae