Faecalibacterium duncaniae as a novel next generation probiotic against influenza

Front Immunol. 2024 Mar 12:15:1347676. doi: 10.3389/fimmu.2024.1347676. eCollection 2024.

Abstract

The gut-lung axis is critical during viral respiratory infections such as influenza. Gut dysbiosis during infection translates into a massive drop of microbially produced short-chain fatty acids (SCFAs). Among them, butyrate is important during influenza suggesting that microbiome-based therapeutics targeting butyrate might hold promises. The butyrate-producing bacterium Faecalibacterium duncaniae (formerly referred to as F. prausnitzii) is an emerging probiotic with several health-promoting characteristics. To investigate the potential effects of F. duncaniae on influenza outcomes, mice were gavaged with live F. duncaniae (A2-165 or I-4574 strains) five days before infection. Supplementation of F. duncaniae was associated with less severe disease, a lower pulmonary viral load, and lower levels of lung inflammation. F. duncaniae supplementation impacted on gut dysbiosis induced by infection, as assessed by 16S rRNA sequencing. Interestingly, F. duncaniae administration was associated with a recovery in levels of SCFAs (including butyrate) in infected animals. The live form of F. duncaniae was more potent that the pasteurized form in improving influenza outcomes. Lastly, F. duncaniae partially protected against secondary (systemic) bacterial infection. We conclude that F. duncaniae might serve as a novel next generation probiotic against acute viral respiratory diseases.

Keywords: Faecalibacterium; SCFAs; influenza; interferons; live biotherapeutic products; microbiota.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Butyrates
  • Dysbiosis / microbiology
  • Faecalibacterium / genetics
  • Fatty Acids, Volatile
  • Humans
  • Influenza, Human*
  • Mice
  • Probiotics*
  • RNA, Ribosomal, 16S / genetics

Substances

  • RNA, Ribosomal, 16S
  • Fatty Acids, Volatile
  • Butyrates

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported in part by the Institut National de la Santé et de la Recherche Médicale (Inserm), Centre National de la Recherche Scientifique (CNRS), University of Lille, Pasteur Institute of Lille. This project was cofounded by the French National Research Agency (Agence Nationale de la Recherche, ANR): AAP générique 2022, ANR-23-CE15-0014-01, GUTSY) (FT), the React-EU COVID2I (programme opérationnel FEDER/FSE/IEJ Nord-Pas de Calais) (FT), and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, 2018/15313-8) (MARV). VS and AB received salary support (PhD fellowship) from Lille University and Fondation pour la Recherche Médicale (FRM, France). PBR received fellowships from FAPESP (2019/14342-7 and 2022/02058-5). JTH is a recipient of an ERC Starting Grant (Metabo3DC-101042759) and received support from ANR (LabEx EGID ANR-10-LABX-0046). FT received salary support from the CNRS.