An ionic liquid-modified PEDOT/Ti3C2TX based molecularly imprinted electrochemical sensor for pico-molar sensitive detection of L-Tryptophan in milk

Food Chem. 2024 Aug 15:449:139114. doi: 10.1016/j.foodchem.2024.139114. Epub 2024 Apr 2.

Abstract

L-Tryptophan (L-Trp) is essential for the human body and can only be obtained externally. It is important to develop a method to efficiently detect L-Trp in food. In this work, ionic liquid (IL) modified poly(3,4-ethylendioxythiophene)/ Titanium carbide (PEDOT/Ti3C2TX) was used as a substrate material to improve detection sensitivity. Molecular imprinted polymers (MIP) film for specific recognition of L-Trp was fabricated on the surface of modified electrodes using electrochemical polymerization. The monitoring results showed that the molecularly imprinted electrochemical sensors (MIECS) exhibited good linearity ranges (10-6 - 0.1 μM and 0.1-100 μM) with a low detection limit (LOD) of 2.09 × 10-7 μM. In addition, the MIECS exhibited remarkable stability, reproducibility, and immunity to interference. A good recovery (93.54-99.59%) was demonstrated in the detection of milk. The sensor was expected to be developed as a highly selective and sensitive portable assay, and applied to the detection of L-Trp in food.

Keywords: Ionic liquid; L-Tryptophan; Molecularly imprinted electro-chemical sensor; PEDOT; Ti(3)C(2)T(X).

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Bridged Bicyclo Compounds, Heterocyclic / chemistry
  • Electrochemical Techniques* / instrumentation
  • Electrochemical Techniques* / methods
  • Electrodes
  • Food Contamination / analysis
  • Ionic Liquids* / chemistry
  • Limit of Detection*
  • Milk* / chemistry
  • Molecular Imprinting*
  • Molecularly Imprinted Polymers / chemistry
  • Polymers* / chemistry
  • Reproducibility of Results
  • Titanium* / chemistry
  • Tryptophan* / analysis
  • Tryptophan* / chemistry

Substances

  • Ionic Liquids
  • Polymers
  • Tryptophan
  • Titanium
  • poly(3,4-ethylene dioxythiophene)
  • Bridged Bicyclo Compounds, Heterocyclic
  • titanium carbide
  • Molecularly Imprinted Polymers