Structure of the K141 capsular polysaccharide produced by Acinetobacter baumannii isolate KZ1106 that carries KL141 at the chromosomal K locus

Anastasiya A Kasimova; Anna S Kolganova; Alexander S Shashkov; Mikhail M Shneider; Yulia V Mikhailova; Andrey A Shelenkov; Anastasiya V Popova; Yuriy A Knirel; Andrey V Perepelov; Johanna J Kenyon

doi:10.1016/j.carres.2024.109097

Structure of the K141 capsular polysaccharide produced by Acinetobacter baumannii isolate KZ1106 that carries KL141 at the chromosomal K locus

Carbohydr Res. 2024 Apr:538:109097. doi: 10.1016/j.carres.2024.109097. Epub 2024 Mar 24.

Affiliations

¹ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
² N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia; D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia.
³ M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 119997, Moscow, Russia.
⁴ Central Scientific Research Institute of Epidemiology, Moscow, Russia.
⁵ State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
⁶ School of Pharmacy and Medical Sciences, Health Group, Griffith University, Gold Coast, Australia; Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia. Electronic address: j.kenyon@griffith.edu.du.

PMID: 38555658
DOI: 10.1016/j.carres.2024.109097

Abstract

The structure of the K141 type capsular polysaccharide (CPS) produced by Acinetobacter baumannii KZ1106, a clinical isolate recovered from Kazakhstan in 2016, was established by sugar analyses and one- and two-dimensional ¹H and ¹³C NMR spectroscopy. The CPS was shown to consist of branched tetrasaccharide repeating units (K-units) with the following structure: This structure was found to be consistent with the genetic content of the KL141 CPS biosynthesis gene cluster at the chromosomal K locus in the KZ1106 whole genome sequence. Assignment of the encoded enzymes allowed the first sugar of the K unit to be identified, which revealed that the β-d-GlcpNAc-(1→3)-d-GlcpNAc bond is the linkage between K-units formed by the Wzy_KL141 polymerase.

Keywords: Acinetobacter baumannii; Capsular polysaccharide; K locus; K141; KL141.

MeSH terms

Acinetobacter baumannii* / chemistry
Acinetobacter baumannii* / genetics
Bacterial Capsules / chemistry
Magnetic Resonance Spectroscopy
Multigene Family
Polysaccharides / analysis
Polysaccharides, Bacterial / chemistry
Sugars

Substances

Polysaccharides
Sugars
Polysaccharides, Bacterial