Efficient degradation and enhanced α-glucosidase inhibitory activity of apricot polysaccharides through non-thermal plasma assisted non-metallic Fenton reaction

Int J Biol Macromol. 2024 May;266(Pt 1):131103. doi: 10.1016/j.ijbiomac.2024.131103. Epub 2024 Mar 22.

Abstract

Dielectric barrier discharge (DBD) was a commonly used non-thermal plasma (CP) technology. This paper aimed to enhance the biological activity of apricot polysaccharides (AP) by using dielectric barrier discharge (DBD-CP) assisted H2O2-VC Fenton reaction for degradation. The degradation conditions were optimized through response surface methodology. The molecular weight (Mw) of degraded apricot polysaccharides (DAP) was 19.71 kDa, which was 7.25 % of AP. The inhibition rate of DAP (2 mg/mL) was 82.8 ± 3.27 %, which was 106.87 % higher than that of AP. DBD-CP/H2O2-VC degradation changed the monosaccharide composition of AP and improved the linearity of polysaccharide chains. In addition, a novel apricot polysaccharide DAP-2 with a Mw of only 6.60 kDa was isolated from DAP. The repeating units of the main chain of DAP-2 were →4)-α-D-GalpA-(1 →, the branch chain was mainly composed of α-D-GalpA-(1 → 2)-α-L-Rhap-(1→ connected to O-3 position →3,4)-α-D-GalpA-(1→. The complex structure formed by the combination of DAP-2 and α-glucosidase was stable. DAP-2 had a higher α-glucosidase binding ability than the acarbose. These results suggested that DAP-2 had the potential to be developed as a potential hypoglycemic functional food and drug.

Keywords: Free radical degradation; Hypoglycemic polysaccharide; Molecular simulation; Optimization.

MeSH terms

  • Glycoside Hydrolase Inhibitors* / chemistry
  • Glycoside Hydrolase Inhibitors* / pharmacology
  • Hydrogen Peroxide* / chemistry
  • Iron / chemistry
  • Molecular Weight
  • Monosaccharides / analysis
  • Monosaccharides / chemistry
  • Plasma Gases* / chemistry
  • Polysaccharides* / chemistry
  • Polysaccharides* / pharmacology
  • Prunus armeniaca* / chemistry
  • alpha-Glucosidases* / chemistry
  • alpha-Glucosidases* / metabolism

Substances

  • Polysaccharides
  • Glycoside Hydrolase Inhibitors
  • Hydrogen Peroxide
  • alpha-Glucosidases
  • Plasma Gases
  • Iron
  • Monosaccharides