Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis

Qiuyan Guo; Minghong Zhao; Qixin Wang; Tianming Lu; Piao Luo; Lin Chen; Fei Xia; Huanhuan Pang; Shengnan Shen; Guangqing Cheng; Chuanhao Dai; Yuqing Meng; Tianyu Zhong; Chong Qiu; Jigang Wang

doi:10.1016/j.biopha.2024.116304

Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis

Biomed Pharmacother. 2024 Apr:173:116304. doi: 10.1016/j.biopha.2024.116304. Epub 2024 Feb 23.

Authors

Qiuyan Guo¹, Minghong Zhao², Qixin Wang¹, Tianming Lu¹, Piao Luo¹, Lin Chen¹, Fei Xia¹, Huanhuan Pang¹, Shengnan Shen¹, Guangqing Cheng¹, Chuanhao Dai¹, Yuqing Meng¹, Tianyu Zhong³, Chong Qiu⁴, Jigang Wang⁵

Affiliations

¹ State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
² First Affiliated Hospital of Gannan Medical University, No. 23, Qingnian Road, Ganzhou, Jiangxi 341000, China.
³ First Affiliated Hospital of Gannan Medical University, No. 23, Qingnian Road, Ganzhou, Jiangxi 341000, China. Electronic address: zhongtianyu@gmail.com.
⁴ State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: cqiu@icmm.ac.cn.
⁵ State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; Department of Pulmonary and Critical Care Medicine, Shenzhen Institute of Respiratory Diseases, and Shenzhen Clinical Research Centre for Geriatrics, Shenzhen People's Hospital; First Affiliated Hospital of Southern University of Science and Technology; Second Clinical Medical College of Jinan University, Shenzhen, 518020, China. Electronic address: jgwang@icmm.ac.cn.

PMID: 38401519
DOI: 10.1016/j.biopha.2024.116304

Abstract

Glycyrrhetinic acid (GA) shows great efficiency against non-small cell lung cancer (NSCLC), but the detailed mechanism is unclear, which has limited its clinical application. Herein, we investigated the potential targets of GA against NSCLC by activity-based protein profiling (ABPP) technology and the combination of histopathology and proteomics validation. In vitro and in vivo results indicated GA significantly inhibited NSCLC via promotion of peroxiredoxin-6 (Prdx6) and caspase-3 (Casp3)-mediated mitochondrial apoptosis. This original finding will provide theoretical and data support to improve the treatment of NSCLC with the application of GA.

Keywords: Activity-based protein profiling; Glycyrrhetinic acid; NSCLC.

MeSH terms

Apoptosis
Carcinoma, Non-Small-Cell Lung* / pathology
Caspase 3
Cell Line, Tumor
Glycyrrhetinic Acid* / pharmacology
Humans
Lung Neoplasms* / pathology
Peroxiredoxin VI / therapeutic use

Substances

Glycyrrhetinic Acid
Caspase 3
Peroxiredoxin VI