Lagerstroemia macrocarpa extract inhibits Th2-mediated STAT6 signaling pathway in human keratinocytes

Fitoterapia. 2024 Apr:174:105859. doi: 10.1016/j.fitote.2024.105859. Epub 2024 Feb 13.

Abstract

In this study, we examined physiological functions as a key material to develop cosmeceuticals using extracts of Lagerstroemia macrocarpa Wall. Ex Kurz (L. macrocarpa). Initially, the L. macrocarpa extract was treated by different concentration and antioxidant assay (DPPH and ABTS) were performed to measure free radical scavenging ability. In the cytotoxicity experiment, the extract was treated into human epidermal keratinocytes with different concentrations to measure cytotoxicity. We found that the extract induces differentiation markers such as keratin (KRT)1, KRT2, KRT9, KRT10 in keratinocytes. Furthermore, the extract significantly induces involucrin (IVL), loricrin (LOR), claudin1 (CLDN1), and filaggrin (FLG) expression, suggesting that it may enhance skin barrier functions. Especially, the extract restored FLG expression inhibited by interleukin (IL)-4/IL-13 in in vitro atopic dermatitis-like model. Therefore, we expect L. macrocarpa extract will be an effective material to develop the therapeutic and cosmeceutical of atopic dermatitis.

Keywords: Antioxidant; Atopic dermatitis; Filaggrin; Keratinocytes; Lagerstroemia macrocarpa; Skin barrier.

MeSH terms

  • Dermatitis, Atopic* / drug therapy
  • Dermatitis, Atopic* / metabolism
  • Humans
  • Intermediate Filament Proteins / metabolism
  • Intermediate Filament Proteins / pharmacology
  • Intermediate Filament Proteins / therapeutic use
  • Keratinocytes
  • Lagerstroemia* / metabolism
  • Molecular Structure
  • Plant Extracts / metabolism
  • STAT6 Transcription Factor / metabolism
  • STAT6 Transcription Factor / pharmacology
  • Signal Transduction

Substances

  • Intermediate Filament Proteins
  • Plant Extracts
  • STAT6 protein, human
  • STAT6 Transcription Factor