Microglial Ffar4 deficiency promotes cognitive impairment in the context of metabolic syndrome

Sci Adv. 2024 Feb 2;10(5):eadj7813. doi: 10.1126/sciadv.adj7813. Epub 2024 Feb 2.

Abstract

Metabolic syndrome (MetS) is closely associated with an increased risk of dementia and cognitive impairment, and a complex interaction of genetic and environmental dietary factors may be implicated. Free fatty acid receptor 4 (Ffar4) may bridge the genetic and dietary aspects of MetS development. However, the role of Ffar4 in MetS-related cognitive dysfunction is unclear. In this study, we found that Ffar4 expression is down-regulated in MetS mice and MetS patients with cognitive impairment. Conventional and microglial conditional knockout of Ffar4 exacerbated high-fat diet (HFD)-induced cognitive dysfunction and anxiety, whereas microglial Ffar4 overexpression improved HFD-induced cognitive dysfunction and anxiety. Mechanistically, we found that microglial Ffar4 regulated microglial activation through type I interferon signaling. Microglial depletion and NF-κB inhibition partially reversed cognitive dysfunction and anxiety in microglia-specific Ffar4 knockout MetS mice. Together, these findings uncover a previously unappreciated role of Ffar4 in negatively regulating the NF-κB-IFN-β signaling and provide an attractive therapeutic target for delaying MetS-associated cognitive decline.

MeSH terms

  • Animals
  • Cognitive Dysfunction* / complications
  • Cognitive Dysfunction* / genetics
  • Humans
  • Metabolic Syndrome* / complications
  • Metabolic Syndrome* / genetics
  • Mice
  • Mice, Knockout
  • Microglia / metabolism
  • NF-kappa B / metabolism
  • Receptors, G-Protein-Coupled* / metabolism
  • Signal Transduction

Substances

  • NF-kappa B
  • FFAR4 protein, mouse
  • Receptors, G-Protein-Coupled