Hsa_circ_0092856 Promoted the Proliferation, Migration, and Invasion of NSCLC Cells by Up-Regulating the Expression of eIF3a

Fuqiang Yuan; Masha Huang; Hanxue Huang; Xiaoyuan Mao; Pan Xie; Xi Li; Yang Gao; Feiyue Zeng; Zhaoqian Liu

doi:10.3390/biomedicines12010247

Hsa_circ_0092856 Promoted the Proliferation, Migration, and Invasion of NSCLC Cells by Up-Regulating the Expression of eIF3a

Biomedicines. 2024 Jan 22;12(1):247. doi: 10.3390/biomedicines12010247.

Authors

Fuqiang Yuan^{1

2}, Masha Huang^{1

2

3}, Hanxue Huang^{1

2}, Xiaoyuan Mao^{1

2}, Pan Xie^{1

2}, Xi Li^{1

2}, Yang Gao⁴, Feiyue Zeng⁵, Zhaoqian Liu^{1

2}

Affiliations

¹ Hunan Key Laboratory of Pharmacogenetics, Department of Clinical Pharmacology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China.
² Institute of Clinical Pharmacology, Engineering Research Center for Applied Technology of Pharmacogenomics of Ministry of Education, Central South University, Changsha 410078, China.
³ Department of Biochemistry and Molecular Cell Biology, College of Basic Medical Sciences, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China.
⁴ Department of Thoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
⁵ Department of Radiology, Xiangya Hospital, Central South University, Changsha 410008, China.

Abstract

Circular RNA (circRNA) plays a very important regulatory role in a variety of human malignancies such as non-small-cell lung cancer (NSCLC). In the current study, we explored the role of hsa_circ_0092856 in the progression of NSCLC. We screened CircRNA from the eIF3a gene in the Circbase database. The biological functions of hsa_circ_0092856 in NSCLC were analyzed via qRT-PCR, a CCK-8 assay, a plate cloning experiment, scratch testing, a transwell chamber experiment, an RNA nuclear mass separation experiment, an RIP experiment, and a Western blot test. The results showed that hsa_circ_0092856 was highly expressed in NSCLC cells, and the knockdown of hsa_circ_0092856 could inhibit the proliferation, migration, and invasion of NSCLC cells. The overexpression of hsa_circ_0092856 has the opposite effect. The expression of eIF3a also changed with the change in hsa_circ_0092856. These results suggest that hsa_circ_0092856 may play a key role in the progression of NSCLC by regulating the expression of eIF3a.

Keywords: NSCLC; circRNA; eIF3a; hsa_circ_0092856; progression.

Grants and funding

82173901/National Natural Science Foundation of China