Cell cycle regulated synthesis of stable mouse thymidine kinase mRNA is mediated by a sequence within the cDNA

Nucleic Acids Res. 1987 Jan 26;15(2):741-52. doi: 10.1093/nar/15.2.741.

Abstract

The cDNA for mouse thymidine kinase (TK) was isolated from a cDNA library in lambda-gt11 and sequenced. It was used as a probe to follow the time course of TK mRNA expression in growth stimulated mouse fibroblasts. Linked to the HSV-TK promoter the cDNA was able to transform LTK-cells to the TK+ phenotype. The transformed cells expressed the TK mRNA and enzyme activity in a growth dependent fashion suggesting that the regulatory element is localized on the cDNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Cycle
  • DNA / metabolism*
  • Embryo, Mammalian
  • L Cells / enzymology
  • Mice
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics*
  • Thymidine Kinase / deficiency
  • Thymidine Kinase / genetics*

Substances

  • RNA, Messenger
  • DNA
  • Thymidine Kinase