CRISPR/Cas9-Mediated Modification of PTP Expression

Abstract

Alteration of protein tyrosine phosphatase (PTP) gene expression is a commonly used approach to experimentally analyze their function in the cell physiology of mammalian cells. Here, exemplified for receptor-type PTPRJ (Dep-1, CD148) and PPTRC (CD45), we provide the CRISPR/Cas9-mediated approaches for their inactivation and transcriptional activation using genome editing. These methods are generally applicable to any other protein of interest.

Keywords: CRISPR; Cas9; Flow cytometry; Gene expression; Immune detection; Nonhomologous end joining; Protein tyrosine phosphatase; Synergistic activation mediators.