Effects of different volumes of ovariectomy on fertility and offspring development of rats

Yu Yang; Xiangyan Ruan; Jiaojiao Cheng; Xin Xu; Alfred O Mueck

doi:10.3389/fendo.2023.1279610

Effects of different volumes of ovariectomy on fertility and offspring development of rats

Front Endocrinol (Lausanne). 2023 Nov 9:14:1279610. doi: 10.3389/fendo.2023.1279610. eCollection 2023.

Authors

Yu Yang¹, Xiangyan Ruan^{1

2}, Jiaojiao Cheng¹, Xin Xu¹, Alfred O Mueck^{1

2}

Affiliations

¹ Department of Gynecological Endocrinology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China.
² Department for Women's Health, University Women's Hospital and Research Center for Women's Health, University of Tuebingen, Tuebingen, Germany.

Abstract

Objective: The purpose of this study was to explore the effect of removal of different volumes of ovarian tissue on fertility and offspring development of SD rats.

Methods: SD rats were randomly divided into 6 groups according to different volumes of ovariectomy: Sham group (n=6), non-ovariectomized; 25%-OVX group (n=6), with half of the left ovary excised; 50%-OVX group (n=5), with the left ovary excised; 75%-OVX group (n=5), with the left ovary and half of the right ovary excised; 87.5%-OVX group (n=6), with the left ovary and three quarters of the right ovary excised; 100%-OVX group (n=6), with bilateral ovaries excised. These female rats (F0) were mated with healthy male rats one and four months after the surgery, and the offspring of F0 rats were named F1^1mon and F1^4mon, respectively. The number of days from mating to delivery and number of live cubs were recorded. At postnatal day 21 (P21), the body weight, length and anogenital distance (AGD) of the cubs were measured.

Results: There were no differences in the number of live cubs between 25%-OVX, 50%-OVX and sham groups. Rats in the 87.5%-OVX group did not give birth at 1 month and 4 months after the operation. When compared with the sham group, the body weight and length of F1^1mon at P21 were increased in 25%-OVX group and 50%-OVX group. However, after the second delivery, we controlled each mother's lactation to no more than eight pups. As a result, there were no differences in the body weight, length and AGD of F1^4mon compared with sham group.

Conclusion: Removal of less than 50% of the ovaries did not affect the fertility of rats and offspring development of rats.

Keywords: fertility; offspring; ovarian function; ovariectomy; premature ovarian insufficient.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Body Weight
Female
Fertility*
Humans
Male
Ovariectomy / adverse effects
Ovary*
Rats
Rats, Sprague-Dawley

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by Natural Science Foundation of Beijing (7202047), Beijing Municipal Administration of Hospitals' Ascent Plan (DFL20181401), the First Batch of Beijing Maternal and Child Health Specialist Demonstration Units “Menopausal Health Specialist”[ (2017) 35]. Beijing Municipal Health Commission, demonstration construction project of Clinical Research ward (No: BCRW202109).