Highly water-soluble and biocompatible hyaluronic acid functionalized upconversion nanoparticles as ratiometric nanoprobes for label-free detection of nitrofuran and doxorubicin

Food Chem. 2024 Apr 16:438:137961. doi: 10.1016/j.foodchem.2023.137961. Epub 2023 Nov 14.

Abstract

Antibiotic detection is crucial and challenging because the widespread consumption of antibiotics has shown extensive harmful effects on food, environment and human health. Here, we propose highly water-soluble and biocompatible hyaluronic acid (HYA) functionalized upconversion nanoparticles (UCNPs) for ratiometric detection of multiple antibiotics. The ultraviolet upconversion luminescence (UCL) from UCNPs was significantly quenched by nitrofurazone (NFZ)/nitrofurantoin (NFT), and blue UCL was quenched by doxorubicin (DOX), while red UCL remained unchanged for internal reference. The UCNPs-HYA nanoprobes exhibit excellently sensitive and selective NFZ, NFT and DOX detection in linear range of 2.5-100 μM, 2.5-80 μM, and 2.5-200 μM with the LOD at 0.28 μM (55 μg/kg), 0.20 μM (48 μg/kg) and 0.17 μM (97 μg/kg), respectively. The nanoprobes achieved detecting real samples of NFZ in lake water, liquid milk and chicken meat with satisfactory results, and UCL bioimaging of DOX in HeLa cells. The UCNPs-HYA ratiometric nanoprobes are promising for food samples detection and potential biosensing in the cellular environment.

Keywords: Antibiotics; Förster resonant energy transfer; Hyaluronic acid; Inner filter effect; Ratiometric nanoprobe; Upconversion nanoparticles.

MeSH terms

  • Anti-Bacterial Agents
  • Doxorubicin
  • HeLa Cells
  • Humans
  • Hyaluronic Acid
  • Nanoparticles*
  • Nitrofurans*
  • Water

Substances

  • Hyaluronic Acid
  • Water
  • Doxorubicin
  • Nitrofurans
  • Anti-Bacterial Agents