Investigation of Cytoplasmic Incompatibility Patterns in Culex pipiens: From Field Samples to Laboratory Isofemale Lines

Alice Namias; Célestine Atyame; Nicole Pasteur; Mathieu Sicard; Mylène Weill

doi:10.1007/978-1-0716-3553-7_2

Investigation of Cytoplasmic Incompatibility Patterns in Culex pipiens: From Field Samples to Laboratory Isofemale Lines

Methods Mol Biol. 2024:2739:17-27. doi: 10.1007/978-1-0716-3553-7_2.

Authors

Alice Namias¹, Célestine Atyame¹, Nicole Pasteur¹, Mathieu Sicard^#¹, Mylène Weill^#²

Affiliations

¹ Institut des sciences de l'évolution Montpellier (ISEM), University of Montpellier, Montpellier Cedex 05, France.
² Institut des sciences de l'évolution Montpellier (ISEM), University of Montpellier, Montpellier Cedex 05, France. mylene.weill@umontpellier.fr.

^# Contributed equally.

PMID: 38006543
DOI: 10.1007/978-1-0716-3553-7_2

Abstract

This protocol describes approaches to qualify Wolbachia-induced cytoplasmic incompatibility (CI) patterns (compatible, uni or bidirectional) in crosses between two or more Culex pipiens isofemale lines, hosting different Wolbachia (wPip) strains. Here, we describe how to (1) collect the larvae in the field and grow them to the adult stage in the insectary, (2) set up isofemale lines in the insectary, (3) genetically characterize the wPip group of these lines, and (4) perform reciprocal crosses to qualify CI.

Keywords: Culex pipiens; Cytoplasmic incompatibility; Field sampling; Isofemale line; Mosquitoes; PCR-RFLP.

MeSH terms

Animals
Culex* / genetics
Cytoplasm
Cytosol