p62 bodies: cytosolic zoning by phase separation

J Biochem. 2024 Feb 25;175(2):141-146. doi: 10.1093/jb/mvad089.

Abstract

Cellular zoning or partitioning is critical in preventing macromolecules from random diffusion and orchestrating the spatiotemporal dynamics of biochemical reactions. Along with membranous organelles, membraneless organelles contribute to the precise regulation of biochemical reactions inside cells. In response to environmental cues, membraneless organelles rapidly form through liquid-liquid phase separation, sequester certain proteins and RNAs, mediate specific reactions and dissociate. Among membraneless organelles, ubiquitin-positive condensates, namely, p62 bodies, maintain cellular homeostasis through selective autophagy of themselves to contribute to intracellular quality control. p62 bodies also activate the anti-oxidative stress response regulated by the KEAP1-NRF2 system. In this review, we present an overview of recent advancements in cellular and molecular biology related to p62 bodies, highlighting their dynamic nature and functions.

Keywords: KEAP1-NRF2 system; autophagy; intracellular quality control; liquid–liquid phase separation; p62 body.

Publication types

  • Review

MeSH terms

  • Autophagy / physiology
  • Intracellular Signaling Peptides and Proteins* / metabolism
  • Kelch-Like ECH-Associated Protein 1 / metabolism
  • NF-E2-Related Factor 2 / metabolism
  • Phase Separation
  • Signal Transduction* / physiology

Substances

  • Kelch-Like ECH-Associated Protein 1
  • Intracellular Signaling Peptides and Proteins
  • NF-E2-Related Factor 2